Yazarlar : Hancock V, Cardigan R, Thomas S.

Yayın : Transfus Med.

Yayın Yılı : 2011

Pubmed Linki : http://www.ncbi.nlm.nih.gov/pubmed/21707796

Konu : Transfüzyon

Literatür İçeriği :

Abstract

Background and Objectives: This study investigated the current UK guidelines for storage and transport of red cell concentrates (RCC) in saline, adenine, glucose and mannitol (SAGM). The guidelines stipulate storage at 2-6 °C but allow exposure to between 1-10 °C core temperature in a single occurrence of less than 5 h and a surface temperature of 2-10 °C for no more than 12 h during transportation. Methods and Materials: Twenty RCC units in SAGM were selected on the day of blood collection (day 0) and in vitro quality was tested pre- and post-temperature deviation at 10 °C and up to day 42 of storage. Each group of 10 RCC units was incubated for either 12 h or for both 5 and 12 h. Results: Haemolysis was below the 0·8% UK limit at day 42 in all units, although there was an unexpected trend towards lower haemolysis in packs incubated for 5 and 12 h rather than just 12 h alone. Supernatant potassium was significantly higher than reference data on day 35 (P < 0·05) with a maximum of 58 mmol L(-1) and day 42 (P < 0·001). All units incubated at 10 °C had comparable levels of adenosine triphosphate and, 2,3-diphosphoglycerate to reference data from previous studies, throughout storage. Conclusion: These results suggest that exposure to 10 °C for 12 h or for 5 and 12 h did not adversely affect in vitro red cell quality for the remainder of the components shelf life.

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