Literatür Detay Bilgisi
Development of A Reverse Genetic System for Infectious Salmon Anemia Virus (ISAV): Rescue of Recombinant Fluorescence Virus Using Salmon ITS-1 Region as a Novel Promoter.

Yazarlar : Toro-Ascuy D1, Tambley C1, Beltran C

Yayın : Appl Environ Microbiol.

Yayın Yılı : 2014

Pubmed Linki : http://www.ncbi.nlm.nih.gov/pubmed/25480750

Konu : Anemi

Literatür İçeriği :  

Abstract

The Infectious salmon anemia (ISA) is a serious disease of marine-farmed Atlantic salmon (Salmo salar) caused by ISA virus (ISAV), belonging to the genus Isavirus, family Orthomyxoviridae. There is an urgent need to understand the virulence factors and pathogenic mechanisms of ISAV and to develop new vaccine approaches. Using a recombinant molecular biology approach, we report for the first time the development of a plasmid based reverse genetic system for ISAV, which includes the use of a novel fish promoter, the Atlantic salmon ITS-1. Salmon cells co-transfected with pSS-URG-based vectors expresings the eight viral RNA segments and four CMV-based vectors that express the four proteins of the ISAV ribonucleoprotein complex allowed the generation of infectious recombinant ISAV (rISAV). We generated three recombinant viruses, a wild type rISAV901_09 and rISAVrS6-NotI-HPR containing a NotI restriction site and rISAVS6/EGFP-HPR harbouring the ORF of enhanced green fluorescent protein (EGFP), both within the HPR region of segment 6. All rescued viruses showed replication activity and cytopathic effect in ASK infected cells. The fluorescent recombinant viruses also showed a characteristic cytopathic effect in salmon cells, and the viruses replicated to a titer of 6.5 x 105PFU/ml, similar to that of the wild-type virus. This novel reverse genetics system offers a powerful tool to study the molecular biology of ISAV and develop new generation of ISAV vaccines to prevent and mitigate ISAV infection, which has had a profound effect on the salmon industry.

Copyright © 2014, American Society for Microbiology. All Rights Reserved.


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