To regenerate discarded lungs that would not normally be used for transplant, ex vivo reseeding following decellularization may produce organs suitable for clinical transplantation and therefore close the donor gap. Organ Regenerative Control Acquisition (ORCA, Harvard Biosciences), a novel bioreactor system that simulates physiological conditions, was used to evaluate a method of rapid decellularization. While most current decellularization methods are 24-72 hours we hypothesized that perfusing porcine lungs with detergents at higher pressures for less time would yield comparable bioscaffolds suitable for future experimentation.Methods involved perfusion of 1% Triton-X 100 (Triton) and 0.1% sodium dodecyl sulfate (SDS) at varied physiological flow rates. Architecture of native and decellularized lungs was analyzed with hematoxylin and eosin staining (H&E), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Dry gas and liquid ventilation techniques were introduced.Our 7-hour decellularization procedure removes nuclear material while maintaining architecture. Bioscaffolds have the microarchitecture for reseeding of stem cells. H&E staining suggested removal of nuclear material, while SEM and TEM imaging demonstrated total removal of cells with structural architecture preserved. This process can lead to clinical implementation, thereby increasing the availability of human lungs for transplantation.