Literatür Detay Bilgisi
Glucose metabolism gene expression patterns and tumor uptake of (18)f-fluorodeoxyglucose after radiation treatment.

Yazarlar : Wilson GD, Thibodeau BJ, Fortier LE et al

Yayın : Int J Radiat Oncol Biol Phys.

Yayın Yılı : 2014

Pubmed Linki : http://www.hematoloji.org.tr/admin/literature.add.php

Konu : Radyasyon Onkolojisi

Literatür İçeriği :

Abstract

PURPOSE:

To investigate whether radiation treatment influences the expression of glucose metabolism genes and compromises the potential use of (18)F-fluorodeoxyglucose positron emission tomography (FDG-PET) as a tool to monitor the early response of head and neck cancer xenografts toradiation therapy (RT).

METHODS AND MATERIALS:

Low passage head and neck squamous cancer cells (UT14) were injected to the flanks of female nu/nu mice to generate xenografts. After tumors reached a size of 500 mm(3) they were treated with either sham RT or 15 Gy in 1 fraction. At different time points, days 3, 9, and 16 for controls and days 4, 7, 12, 21, 30, and 40 after irradiation, 2 to 3 mice were assessed with dynamic FDG-PET acquisition over 2 hours. Immediately after the FDG-PET the tumors were harvested for global gene expression analysis and immunohistochemical evaluation of GLUT1 and HK2. Different analytic parameters were used to process the dynamic PET data.

RESULTS:

Radiation had no effect on key genes involved in FDG uptake and metabolism but did alter other genes in the HIF1α and glucose transport-related pathways. In contrast to the lack of effect on gene expression, changes in the protein expression patterns of the key genes GLUT1/SLC2A1 and HK2 were observed after radiation treatment. The changes in GLUT1 protein expression showed some correlation with dynamic FDG-PET parameters, such as the kinetic index.

CONCLUSION:

(18)F-fluorodeoxyglucose positron emission tomography changes after RT would seem to represent an altered metabolic state and not a direct effect on the key genes regulating FDG uptake and metabolism.

Copyright © 2014 Elsevier Inc. All rights reserved.


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