Yazarlar : Marchal JA, Picón M, Perán M, Bueno C, Jiménez-Navarro M, Carrillo E, Boulaiz H, Rodríguez N, Alvarez P, Menendez P, de Teresa E, Aránega A.
Yayın : Stem Cells Dev.
Yayın Yılı : 2011
Pubmed Linki : http://www.ncbi.nlm.nih.gov/pubmed/21542697
Konu : Rejeneratif Tıp
Literatür İçeriği : Aims─Endothelial progenitor cells (EPC) represent a relatively rare cell population, and expansion of sufficient cell numbers remains a challenge. Nevertheless, human adipose-derived stem cells (hASC) can be easily isolated and possess the ability to differentiate into endothelial cells. Here, we propose the isolation and characterization of multipotent endothelial-like cells (ME-LC) with the capacity to maintain their vascular progenitor properties for long periods of time. Methods and Results─hASC were isolated from lipoaspirates and cultured through distinct consecutive culture stages for two months to enrich ME-LC: first in DMEM-FBS (stage I), followed by a stage of culture in absent of FBS (stage II), a culture in SFO3 medium (stage III) and finally, the culture of ME-LC into collagen IV-coated flasks in EGM-2 medium (stage IV). ME-LC display increased expression levels of endothelial and hematopoietic lineage markers (CD45, KDR and CXCR4) and EPC markers (CD34 and CD133) whereas the expression of CD31 was barely detectable. RT-PCR assays showed expression of genes involved in early stages of EPC differentiation and decreased expression of genes associated to differentiated EPC (TIE-2, DLL4 and FLT-1). ME-LC formed capillary-like structures when grown on Matrigel, secreted increased levels of SDF-1 and showed the ability to migrate attracted by SDF-1, VEGF and HGF cytokines. Importantly, ME-LC retained the capacity to differentiate into cardiomyocyte-like cells. Conclusions─We present a simplified and efficient method to generate large numbers of autologous ME-LC from lipoaspirates-derived hASC, opening up potential cell-based therapies for cardiovascular regenerative medicine.
| Sunumlar | Videolar | Olgu Tartışması |
